Test for neutralizers: Neutralizers like sodium bicarbonate, sodium hydroxide, sodium carbonate or hydrated lime are often added to milk. Usually they are added to milk to increase the lactometer reading. The neutralizers have influence on the values of titratable acidity and pH of milk that is why they must be checked in milk sample. The impregnate test zone with chromogens change color from orange to orange red in the presence of sodium carbonate/sodium bicarbonate/sodium hydroxide. If the color of neutralizers test changes from orange to orange-red, this means that the milk was adulterated with sodium carbonate/sodium bicarbonate/sodium hydroxide (> 0.2% NaHCO3 ; > 0.1% Na2CO3 and > 0.1%NaOH) and the result is positive (+), (abnormal value). This test will be effective only if the neutralizers are present in milk. If the added neutralizers are nullified by the developed acidity, then this test will be negative (-). In that case, the alkaline condition of the milk for the presence of soda ash has to be estimated by another method. The neutralizers content in milk should be estimated by comparison with color scale at 60s.